Quantitative Analysis of Total Amino Acid in Barley Leaves under Herbicide Stress Using Spectroscopic Technology and Chemometrics

Visible and near infrared (Vis/NIR) spectroscopy were employed for the fast and nondestructive estimation of the total amino acid (TAA) content in barley (Hordeum vulgare L.) leaves. The calibration set was composed of 50 samples; and the remaining 25 samples were used for the validation set. Seven different spectral preprocessing methods and six different calibration methods (linear and nonlinear) were applied for a comprehensive prediction performance comparison. Successive projections algorithm (SPA) and regression coefficients (RC) were applied to select effective wavelengths (EWs). The results indicated that the latent variables-least-squares-support vector machine (LV-LS-SVM) model achieved the optimal performance. The prediction results by LV-LS-SVM with raw spectra were achieved with a correlation coefficients (r) = 0.937 and root mean squares error of prediction (RMSEP) = 0.530. The overall results showed that the NIR spectroscopy could be used for determination of TAA content in barley leaves with an excellent prediction precision; and the results were also helpful for on-field monitoring of barley growing status under herbicide stress during different growth stages.